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Image Search Results
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Changes in Elastic Moduli of Fibrin Hydrogels Within the Myogenic Range Alter Behavior of Murine C2C12 and Human C25 Myoblasts Differently
doi: 10.3389/fbioe.2022.836520
Figure Lengend Snippet: Primer sequences, primer concentrations and thermal profiles used for qPCR.
Article Snippet: Blocking was performed with PBS/T-1% bovine serum albumin (BSA) (w/v) at room temperature for 1 h. The primary antibody targeting all
Techniques:
Journal: Autophagy
Article Title: USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy
doi: 10.1080/15548627.2021.1963155
Figure Lengend Snippet: USP19 deficiency in macrophages enhanced VSV-induced type I interferon production and antiviral response by suppressing CMA-mediated TBK1 degradation. (A and B) qPCR analysis of Ifna4 (A) and Ifnb1 (B) expression in Usp19flox/flox and usp19flox/flox Lyz2-Cre peritoneal macrophages infected with VSV (MOI = 1) for the indicated times with or without VER-155,008 (5 μM) pretreatment. (C and D) qPCR analysis of Ifna4 (C) and Ifnb1 (D) expression in Usp19flox/flox and usp19flox/flox Lyz2-Cre peritoneal macrophages transfected with poly(I:C) (1 μg/mL) for the indicated hours with or without pretreatment with VER-155,008 (5 μM). (E and F) qPCR analysis of Ccl5, Cxcl10 (E), Isg15, Ifit2, Ifit1, and Mx1 expression (F) in Usp19flox/flox and usp19flox/flox Lyz2-Cre peritoneal macrophages infected with VSV (MOI = 1) for 12 h with or without pretreatment with VER-155,008 (5 μM). (G) Immunoblot analysis of OSA1, EIF2AK2, and LAMP2A expression in Usp19flox/flox and usp19flox/flox Lyz2-Cre peritoneal macrophages infected with VSV (MOI = 1) for the indicated durations. (H and I) Usp19flox/flox and usp19flox/flox Lyz2-Cre peritoneal macrophages were infected with VSV-GFP for 12 h with or without VER-155,008 (5 μM) pretreatment, and images were captured under a fluorescence microscope (H). The percentage of GFP+ cells was determined via flow cytometry (I). (J) qPCR analysis of VSV replication in Usp19flox/flox and usp19flox/flox Lyz2-Cre peritoneal macrophages infected with VSV (MOI = 1) with or without VER-155,008 (5 μM) pretreatment. (K) Determination of VSV titers in supernatants via a TCID50 assay of Usp19flox/flox and usp19flox/flox Lyz2-Cre peritoneal macrophages infected with VSV with or without VER-155,008 (5 μM) treatment. (L) Immunoblot analysis of the indicated proteins in Usp19flox/flox and usp19flox/flox Lyz2-Cre peritoneal macrophages infected with VSV (MOI = 1) for the indicated durations. (M) Immunoblot analysis of STAT1 and STAT2 phosphorylation in Usp19flox/flox and usp19flox/flox Lyz2-Cre peritoneal macrophages infected with VSV (MOI = 1) for the indicated hours. The data are representative of three independent experiments. Scale bars: 100 μm. Error bars show the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 using Student’s t test.
Article Snippet: Antibodies specific to p-IRF3 (4947), IRF3 (4302), p-TBK1 (5483), TBK1 (3504), p-STAT1 (9167),
Techniques: Expressing, Infection, Transfection, Western Blot, Fluorescence, Microscopy, Flow Cytometry, TCID50 Assay, Phospho-proteomics